Hello, I was wondering if anyone has tried FRET in flow to more than one acceptor and, if so, to what dyes. The idea is a CFP or eGFP expressed protein will be bound to two or three other molecules which each have their own acceptor dye. The relative abundance of the proteins is high and we have done single combinations of the CFP protein with each acceptor molecule but would like to squeeze in all three chaperone molecules at the same time. Any thoughts, ideas, references and working protocols are most welcome. Thanks. -Bill ********************************************** Bill Hyun, Director Laboratory for Cell Analysis, Box 0808 UCSF Comprehensive Cancer Center San Francisco, CA 94143-0808 (415) 476-2632 Office (415) 476-8218 FAX hyun@cc.ucsf.edu http://cancer.ucsf.edu/lca/index.phpReceived on Mon Jul 14 13:58:00 2008
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