Hi, I have a compensation problem. I use Pacific Blue, FITC, PE, PerCP and APC for my Antibody panels and I use FACS Aria 1 for analysis. I do compensation using BD CompBeads according to BD protocol and do auto compensation in FACS Diva. My problem is I get very high compensation due to bleeding as shown below. PerCP-PE 70.35% APC-PerCP 102.83% Other seems OK. I use following detectors and lasers as set by our flow manager. 405 Pacific Blue 450/50 488 FITC 530/30 with 505LP 488 PE 575/25 with 550LP 488 PerCP 710/50 with 685LP 633 APC 660/20 I tried, for PerCP the 660/20 with 635LP in the blue laser, then it looked worse. Any suggestions? Thanks Viskam Viskam Wijewardana Center for Vaccine Research 9051 Biomedical Sciences Tower 3 University of Pittsburgh 3501 Fifth Avenue Pittsburgh, Pennsylvania 15261 Ph 412 383 9057 Fax 412 624 4577Received on Fri Jul 11 14:18:00 2008
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