Dear Flowers We have been subscribing to CAP PNH EQA for about 1 year. Have encountered a problem in results for recent CAP PNH EQA that we are not sure what to do. In the recent March CAP PNH EQA, for PNH 01 sample, we could identify only 1 single population using IOTest CD59 (Clone P282E) although there is supposed to be 2 populations.. The reason is due to the antibody clone we use since with BD-CD59 or Caltag-CD59 there is clear separation of PNH cells ie. 2 populations seen in contrast to IOTest-CD59 which gives only 1 single population. We do no lyse, no wash staining, using IOTest CD59 (Clone P282E) . Our normal approach for PNH screening is CD16/55/59 on granulocytes and CD55/59 on rbcs and we use IO reagents since we have use of Coulter machine on reagent contract basis. Seems to be working fine past few years and we validate the reagents for each change of lot and ensure they are used before expiry period. In view of recent CAP results, we are wondering if we shoulcl change our practice or CD59 antibody clones we use Just wonder what is anyone’s experience and response ?? Would appreciate your advice and input Many thanks LC Lim, MD Consultant Haematologist, Dept of Hematology (Clinical and Laboratory) Singapore General Hospital Singapore _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#DisclaimerReceived on Fri Jul 11 12:38:00 2008
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