ki-67 stain permeabilization?

Hi Alessandro.
 
We tested various fix&perm methods for staining of different intracellular antigens- also Ki-67- and compared different commercial available fix&perm kits with self-made fix&perm solutions.
Our results obtained that for Ki-67 either PFA fixation in combination with saponin permeabilisation or the BD fix&perm kit gave the best results of the tested fix&perm methods- at least in the porcine system.
Specified procedures and staining results of surface and intracellular antigens were published in Vet. Immunol Immunopathol. 2008 Feb 15;121(3-4):251-9.
 
Good luck, Tobias
 
Dipl.-Biol. Tobias Kaeser
University of Veterinary Medicine Vienna
Department of Pathobiology
Institute for Immunology
Veterinaerplatz 1
1210 Vienna
Austria
Tel: +43-1-25077-2753
email: tobias.kaeser@vu-wien.ac.at 



From: alessandro serra [mailto:serra@drfz.de] 
Sent: den 1 juli 2008 13:09
To: cyto-inbox
Subject: ki-67 stain permeabilization?



Dear All



Can anybody suggest which is the best method to permeabilize cells in order to obtain the most reliable staining with Ki-67 Ab from BD?

Thank you very much



Dr. Alessandro Serra

Cell Biology Group

Deutsches Rheumaforschungszentrum (DRFZ) Berlin

Charitéplatz 1

10117 Berlin



Phone: +49-30-28460 765

Fax: +49-30-28460 603

Email: serra@drfz.de



___________________________________________

Samuel Lundin, Docent in Immunology,

Dept. of Microbiology and Immunology and

MIVAC - Mucosal Immunobiology and Vaccine Center,

Institute of Biomedicine, Göteborg University,

Box 435; 40530 Göteborg; Sweden

Phone: +46-31-7866207