Hi Sheree, As long as your polyclonal anti-IgG reacts with both the heavy and light chain it will cross reacts with IgM. The light chain are similar in all isotypes. So you must buy a heavy chain specific reagents. We had good luck in the past with those from Southern Biotech. However we did not test their reagents against mouse. George University of Cincinnati & Shriners Hospitals for Children.
attached mail follows:
for <cytometry@flowcyt.cyto.purdue.edu>; Thu, 3 Jul 2008 11:30:09 +0930 (CST)' Received: from EMSGM302.sagemsmrd01.sa.gov.au (emsgm302.sagemsmrd01.sa.gov.au [10.9.18.85]) by adl0741.systems.sa.gov.au INCOMING (8.12.10/8.12.10) with ESMTP id m63209aE020110 for <cytometry@flowcyt.cyto.purdue.edu>; Thu, 3 Jul 2008 11:30:09 +0930 (CST)' Received: from hadaltmail.althad.sa.gov.au (10.18.2.3) by EMSGM302.sagemsmrd01.sa.gov.au (10.9.18.85) with Microsoft SMTP Server id 8.1.263.0; Thu, 3 Jul 2008 11:29:57 +0930 X-MimeOLE: Produced By Microsoft Exchange V6.5 Content-Class: urn:content-classes:message MIME-Version: 1.0 Content-Type: multipart/alternative; boundary="----_=_NextPart_001_01C8DCB0.857D8548" Subject: anti-IgG Date: Thu, 3 Jul 2008 11:30:08 +0930 Message-ID: <B60267BF141079468B739731138FFC34039B6D6E@hadaltmail.althad.sa.gov.au> Thread-Topic: anti-IgG Thread-Index: AcjcsITn6sCPjaHdQZ2Zp5PijiEK9g== From: "Bailey, Sheree (FMC)" <Sheree.Bailey@fmc.sa.gov.au> Precedence: list List-Id: Cytometry Mailing List <cyto-list.cyto.purdue.edu> To: cyto-inbox X-Spam-Score: -4.687 () BAYES_00,HTML_60_70,HTML_MESSAGE X-Scanned-By: MIMEDefang 2.39 X-PMFLAGS: 570950016 0 1 87019.cnm X-Junkmail-Status: score=10/50, host=mirapoint2.uc.edu X-Junkmail-SD-Raw: score=unknown, refid=str=0001.0A090204.486D1770.01A8,ss=1,fgs=0, ip=128.210.61.51, so=2008-05-01 23:44:25, dmn=5.4.3/2008-02-01 X-Junkmail-IWF: false ------_=_NextPart_001_01C8DCB0.857D8548 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable I am in search of an unconjugated polyclonal F(ab')2 fragment of anti-human IgG (H+L) antibody. I have tried Dako rabbit anti-human IgG (Cat. No. A0423), Sigma Goat anti-human IgG (Cat. No. 027K4827) and Rocklands Goat anti-human IgG (Cat. No. 709-1116), however these antibodies either bind IgM or cross react with our mouse monoclonal antibodies. Can anyone recommend an unconjugated polyclonal F(ab')2 fragment of anti-human IgG (H+L) that they have used successfully with minimal species cross reactivity and that does not bind IgM. =20 Sheree Bailey Senior Medical Scientist Dept. of Immunology, Allergy and Arthritis Flinders Medical Centre Bedford Park SA 5042 AUSTRALIA =20 ------_=_NextPart_001_01C8DCB0.857D8548 Content-Type: text/html; charset="us-ascii" Content-Transfer-Encoding: quoted-printable <html xmlns:v=3D"urn:schemas-microsoft-com:vml" = xmlns:o=3D"urn:schemas-microsoft-com:office:office" = xmlns:w=3D"urn:schemas-microsoft-com:office:word" = xmlns:st1=3D"urn:schemas-microsoft-com:office:smarttags" = xmlns=3D"http://www.w3.org/TR/REC-html40"> <head> <META HTTP-EQUIV=3D"Content-Type" CONTENT=3D"text/html; = charset=3Dus-ascii"> <meta name=3DGenerator content=3D"Microsoft Word 11 (filtered medium)"> <o:SmartTagType = namespaceuri=3D"urn:schemas-microsoft-com:office:smarttags" name=3D"country-region"/> <o:SmartTagType = namespaceuri=3D"urn:schemas-microsoft-com:office:smarttags" name=3D"place"/> <!--[if !mso]> <style> st1\:*{behavior:url(#default#ieooui) } </style> <![endif]--> <style> <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {margin:0cm; margin-bottom:.0001pt; font-size:12.0pt; font-family:"Times New Roman";} a:link, span.MsoHyperlink {color:blue; text-decoration:underline;} a:visited, span.MsoHyperlinkFollowed {color:purple; text-decoration:underline;} span.EmailStyle17 {mso-style-type:personal-compose; font-family:Arial; color:windowtext;} @page Section1 {size:595.3pt 841.9pt; margin:72.0pt 90.0pt 72.0pt 90.0pt;} div.Section1 {page:Section1;} --> </style> </head> <body lang=3DEN-AU link=3Dblue vlink=3Dpurple> <div class=3DSection1> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'>I am in search of an unconjugated polyclonal = F(ab’)2 fragment of anti-human IgG (H+L) antibody. I have tried Dako rabbit = anti-human IgG (Cat. No. A0423), Sigma Goat anti-human IgG (Cat. No. 027K4827) and Rocklands Goat anti-human IgG (Cat. No. 709-1116), however these = antibodies either bind IgM or cross react with our mouse monoclonal antibodies. Can = anyone recommend an unconjugated polyclonal F(ab’)2 fragment of = anti-human IgG (H+L) that they have used successfully with minimal species cross = reactivity and that does not bind IgM.<o:p></o:p></span></font></p> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'><o:p> </o:p></span></font></p> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'>Sheree Bailey<o:p></o:p></span></font></p> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'>Senior Medical Scientist<o:p></o:p></span></font></p> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'>Dept. of Immunology, Allergy and = Arthritis<o:p></o:p></span></font></p> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'>Flinders Medical Centre<o:p></o:p></span></font></p> <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'>Bedford Park SA 5042<o:p></o:p></span></font></p> <p class=3DMsoNormal><st1:place w:st=3D"on"><st1:country-region = w:st=3D"on"><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt;font-family:Arial'>AUSTRALIA</span></font></st1= :country-region></st1:place><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt;font-family:Arial'><o:p></o:p></span></font></p= > <p class=3DMsoNormal><font size=3D2 face=3DArial><span = style=3D'font-size:10.0pt; font-family:Arial'><o:p> </o:p></span></font></p> </div> </body> </html> ------_=_NextPart_001_01C8DCB0.857D8548--Received on Fri Jul 4 21:18:00 2008
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