Hi Mark, We are using new filters in our FacsAria to perform EGFP-YFP separation. For EGFP we use a 495LP and a 510/20 BP For YFP we use a 527LP and a 550/30 BP We use a compensation about 20-30% for each one. The results are quite good. We acquire the filters at Omega Filters. Alexandre Bote Tronchoni UCTS - Unitat Científico-Tècnica de Suport Plataforma de Citòmica Telf: +34 934894179 Fax: +34 934894185 Institut de Recerca Hospital Universitari de la Vall d'Hebron Passeig de la Vall d'Hebron 119-129 08035 Barcelona http://www.ir.vhebron.net/easyweb_irvh/default.aspx?language=ca Si té solució, de què et queixes? Si no té solució, de què et queixes? On Thu, Jul 3, 2008 at 14:35, Mark Shlomchik <mark.shlomchik@yale.edu> wrote: > Hi All, > > Has anyone been separating GFP and YFP on the LSRII/Aria? > > I am interested in suggestions for filter sets that will minimize > compensation and maximize sensitivity (aren't we all?) In any case, if you > can share your experience that would be helpful before we order filters. > > Thanks, > > Mark > -- > Mark Shlomchik, MD, PhD > Professor of Laboratory Medicine > and Immunobiology > Yale University School of Medicine >Received on Fri Jul 4 20:58:00 2008
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