Mark: We have been doing this extensively on both an LSR-II and FACSAria in multichromatic work (8+ colors) For example, Neering et al., Blood 110:2578-2585 (2007) (http://bloodjournal.hematologylibrary.org/cgi/content/full/110/7/2578) We use: LP BP YFP 525LP 530/30 GFP 502LP 510/20 Regards Tim Tim Bushnell, Ph.D. Research Assistant Professor, Pediatrics and Oncology Co-Director, URMC Flow Cytometry Facility Office: 585-273-5535 Lab: 585-273-1361 Cell: 585-690-5157 Fax: 585-276-0233 http://www.urmc.rochester.edu/flow-core/ http://www.urmc.rochester.edu/wnyfug/ On 7/3/08 8:35 AM, "Mark Shlomchik" <mark.shlomchik@yale.edu> wrote: > Hi All, > > Has anyone been separating GFP and YFP on the LSRII/Aria? > > I am interested in suggestions for filter sets that will minimize > compensation and maximize sensitivity (aren't we all?) In any case, > if you can share your experience that would be helpful before we > order filters. > > Thanks, > > MarkReceived on Fri Jul 4 19:38:00 2008
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