RE: GFP and YFP on the LSRII/Aria

There are special filter sets for GFP and YFP. You need to split the
signal at 525nM (where you would normally read GFP) and read GFP at
510nM and YFP at 550 nM. Omega Optical is one place that sells them. 


Dan Rosson PhD 
University of Tennessee 

-----Original Message-----
From: Mark Shlomchik [mailto:mark.shlomchik@yale.edu] 
Sent: Thursday, July 03, 2008 7:35 AM
To: cyto-inbox
Subject: GFP and YFP on the LSRII/Aria

Hi All,

Has anyone been separating GFP and YFP on the LSRII/Aria?

I am interested in suggestions for filter sets that will minimize
compensation and maximize sensitivity (aren't we all?) In any case, if
you can share your experience that would be helpful before we order
filters.

Thanks,

Mark
--
Mark Shlomchik, MD, PhD
Professor of Laboratory Medicine
and Immunobiology
Yale University School of Medicine