DNA content of bacilli

Looking for some help here. Although I have a lot of experience in
running DNA content on mammalian cells I have been asked to look into
staining bacilli. The samples I will recieve will have differing DNA
content, which I need to use to put a number to each cell type. The
method suggested was with Hoecht 33342, but we do not have a UV laser.
The methods I have cobbled together are 1) Fix and stain with PI, or 2)
Stain with syto-9 (part of Invitrogen's Live/Dead discrimination kit. I
just wondered if anyone did this sort of thing routinely, and if they
had a protocol I might try.
Thanking in advance for your input,
Eric
 
Eric Miller
Senior Flow Cytometry Analyst
Charles River laboratories
Tranent
East Lothian
EH33 5NJ
Tel 01875614545
 
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