Dear Colleagues, We have a user, who wants to measure the DNA of parasites (e.g C.elegans) using FACS Calibur or LSRII. However, when we run the sample we don't appear to detect any DNA profile, although the QC check samples (chicken erythrocyte nuclei and calf thymocyte nuclei from BD) show nice DNA and linear profiles. I would like to know if anyone has a protocol for preparing of nematode cells for FACS and can provide any suggestions for the setting of above instruments. Any guidelines would be helpful in this procedure. Thanks very much for your help. All the best, Raif ---------------------------------- Dr. Raif Yücel Flow Cytometry Facility Manager School of Medicine Institute of Medical Sciences Room 4.63 University of Aberdeen Foresterhill Aberdeen AB25 2ZD Scotland-UK Telephone: +44 (0)1224 555756 Fax: +44 (0)1224 555955 E-mail: raif.yucel@abdn.ac.uk Webpage: http://www.abdn.ac.uk/ims/flowcyto/index.shtml The University of Aberdeen is a charity registered in Scotland, No SC013683--_000_9E465A5DA42E404C936DA93EFD2D649501928BF9VMAILAuoaabdnac_--Received on Wed Jun 18 12:38:00 2008
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