autofluorescence and compensation

From: Eric Shaw <eseric47@googlemail.com>
Date: Wed Jun 11 2008 - 12:20:02 EDT
Hi Flowers,
I have a question.
I have a tissue dissagregation which will have lots of dead cells and
various cell types which most likely have different amounts of
autofluorescence. I know I need to use single stained controls (unstained,
7AAD, FITC,PE,APC) but wont my compensation be inaccurate for FITC/PE/APC
since I am compensating for the whole population- live and dead? And what
about the different cell types (epi's, tissue macrophages etc) that can give
differing amounts of autoflorescence? I compensate with my R1 gate  off
FSC/SSC and I am using a FACS Calibur. All replies will be appreciated.

Thanks,

Eric Shaw
Received on Thu Jun 12 14:58:00 2008

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