Hi Flowers, I have a question. I have a tissue dissagregation which will have lots of dead cells and various cell types which most likely have different amounts of autofluorescence. I know I need to use single stained controls (unstained, 7AAD, FITC,PE,APC) but wont my compensation be inaccurate for FITC/PE/APC since I am compensating for the whole population- live and dead? And what about the different cell types (epi's, tissue macrophages etc) that can give differing amounts of autoflorescence? I compensate with my R1 gate off FSC/SSC and I am using a FACS Calibur. All replies will be appreciated. Thanks, Eric ShawReceived on Thu Jun 12 14:58:00 2008
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