Hi Rudjer, I've always found the Southern Biotechs' IgG-specific antibodies are highly specific and have minimal cross-reaction with other species or isotypes. I've never tried their anti-rat IgG2a, but they have it in a few useful conjugations: FITC,PE,Cy5 and Biotin (go to http://www.southernbiotech.com/cgi/productsearch.asp and search for "anti-rat Igg2a" (note that "AP" is alkaline phosphatase in their catalogue not APC, so not so useful for flow), Cheers Ray Ray Hicks Cytek Development Inc http://www.cytekdev.com tel: +44 (0)208 1337 968 (UK/Europe) fax: +44(0)208 5889 004 skype: ray.hicks.cytek -----Original Message----- From: Rudjer Novak [mailto:rnovak@pharma.hr] Sent: 26 May 2008 10:42 To: cyto-inbox Subject: IgG allotypes dear flowers, I use a primary (rat) IgG2a antibody and need a secondary, but I can't find an IgG2a specific one. The best I could find was a polyclonal anti rat IgG Ab and a H+L specific anti rat IgG Ab (which sound the same to me?). Is that gonna work? Would you prefer any of the mentioned secondaries? What is the structural difference between IgG allotypes? thanks for your time! rudjer ________________________ Rudjer Novak, dipl. ing. Farmaceutsko-biokemijski fakultet Zavod za biokemiju i molekularnu biologiju A, Kovacica 1 10000 Zagreb tel: 095 804 3102 ________________________ Rudjer Novak, B.Sc. University of Zagreb Faculty of Pharmacy and Biochemistry A. Kovacica 1, Zagreb CroatiaReceived on Wed May 28 11:38:00 2008
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST
