RE: Aria and 130 nozzle

To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
From: "Greg Veltri" <Greg.Veltri@cancer.org.uk>
Date: 05/10/2008 04:30PM
Subject: RE: Aria and 130 nozzle

Hi Barb,

I have a 130um nozzle and using a frequency between 17-18 at 12 psi works well for us.  We can see the break-off and have no issues sorting.  You may need to place the attenuation on.  The key is to make sure you get the air out of system (let it sit 30 -60 mins before accudrop) and start with a full PBS container. 

Good luck,

Greg

____________________________________________________________
Gregory Veltri
Head of Flow Cytometry
Cancer Research UK
Cambridge Research Institute
Robinson Way
Cambridge
CB2 0RE
01223 404170

http://science.cancerresearchuk.org/sci/flow_cytometry/



-----Original Message-----
From: Barbara Joan Taylor [ mailto:taylorba@mail.nih.gov ]
Sent: Thu 5/8/2008 8:44 PM
To: cyto-inbox Subject: Aria and 130 nozzle

Aria users

I am going to try the 130 nozzle for the first time - can I get some
suggestions for a starting point for sheath pressure and frequency?

Thanks

Barb
Barbara J Taylor
Facility Manager, FACS Core
CCR, NCI, NIH
Bldg 37 Room 6008
37 Convent Dr
Bethesda, MD 20892-4255
taylorba@mail.nih.gov
ph: 301-594-6892
fax: 301-496-8709