the way around this is to first sort your cells as live cells and then do the BrDU procedure. just be sure to optimize your BrDU for very small #s of cells first. ======================================================= Rachel M. Gerstein, Ph.D. Associate Professor Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) -----Original Message----- From: Anupama Ahuja [mailto:anupama.ahuja@yale.edu] Sent: Tue 5/13/2008 2:10 PM To: cyto-inbox Subject: BrdU: Ethanol fixation and compatibility with fluorophores Hello I have been using the BrdU staining protocol using Ethanol, followed by PFA/tween-20 for a while now. I know that APC and APC-cy7 Abs don't do well with this procedure. I was wondering if anyone knows specifically how this protocol affects PerCP, PEcy5.5, PerCP-cy5.5, PE-cy5 and/or PE-texasred Abs? I am practically running out on colors even for our LSR2 and have to use one of the above-mentioned colors! I really appreciate the help.... Anu -- Anupama Ahuja, Ph. D. Associate Research Scientist Dept. of Laboratory Medicine Yale University School of Medicine 1 Gilbert St. TAC S541 New Haven, CT 06510 Ph: (203)-785-6557 Fax: (203)-785-5415Received on Thu May 15 16:58:00 2008
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