Ovine trophoblasts

Dear Flow-ers,

One of my costumers is trying to purify ovine trophoblasts. They 
produce interferon tau, placental lactogen and progesterone but he's 
having troubles finding reagents. We can get rid of fibroblasts using 
a fibroblast marker. But I would like to know if there is a positive 
marker for this group of cells, which marker I should use? He doesn't 
know the size of the cells, and he told me that the literature on 
ovine trophs is limited. I really apreciate any help on this.

Kind regards,

Alfonso

------------------------------
 Dr. Alfonso Blanco Fernández

 Flow Cytometry Core Facilities
 UCD - Conway Institute of Biomolecular & Biomedical Research
 University College Dublin
 Belfield, Dublin 4
 IRELAND

 T: 00353(0)1 716 6836/6947

----- Original Message -----
From: "Kaluarachchi, Warna D." <wdkaluar@UTMB.EDU>
Date: Wednesday, May 14, 2008 0:45 am
Subject: FW: FACS Quanta SC
To: cyto-inbox

> This is what I received for the question I asked. Please see below.
> Thanks for responding. 
> Warna
> 
> ________________________________
> 
> From: Langsdorf, Chris [mailto:chris.langsdorf@invitrogen.com] 
> Sent: Thursday, May 08, 2008 2:59 PM
> To: cyto-inbox
> Subject: RE: FACS Quanta SC
> 
> 
> We just had the same problem and the engineer said that our laser was
> out of alignment. 
> 
> ________________________________
> 
> From: Kaluarachchi, Warna D. [mailto:wdkaluar@UTMB.EDU] 
> Sent: Wednesday, May 07, 2008 11:59 AM
> To: cyto-inbox
> Subject: FACS Quanta SC
> 
> 
> Hi All,
> We recently bought a FACS Quanta SC mechine. If you have this mechine
> please let me know, I am having difficulties getting the cells on the
> scatter plot. The cells are close to the bottom of the plot. I try
> changing SS and EV, but nothing comes up. I see cells under the
> microscope. We are still learing this mechine. I operate BD FACS 
Aria,
> and BD FACS Canto, I don't have any problem like this. I really
> appreciate if you can give me any tips. Thanks.
> Warna
>