This is what I received for the question I asked. Please see below. Thanks for responding. Warna ________________________________ From: Langsdorf, Chris [mailto:chris.langsdorf@invitrogen.com] Sent: Thursday, May 08, 2008 2:59 PM To: cyto-inbox Subject: RE: FACS Quanta SC We just had the same problem and the engineer said that our laser was out of alignment. ________________________________ From: Kaluarachchi, Warna D. [mailto:wdkaluar@UTMB.EDU] Sent: Wednesday, May 07, 2008 11:59 AM To: cyto-inbox Subject: FACS Quanta SC Hi All, We recently bought a FACS Quanta SC mechine. If you have this mechine please let me know, I am having difficulties getting the cells on the scatter plot. The cells are close to the bottom of the plot. I try changing SS and EV, but nothing comes up. I see cells under the microscope. We are still learing this mechine. I operate BD FACS Aria, and BD FACS Canto, I don't have any problem like this. I really appreciate if you can give me any tips. Thanks. WarnaReceived on Tue May 13 16:38:00 2008
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