Dear Colleagues Bone marrow aspiration is really aggressive, and none of us could put ourselves in the patients' shoes to understand how painful it is. There are actually a plenty of cells in each micro liter of B.M samples; I myself use only 10 ul for each tube and some times even less (5 ul). In this case if you have 500ul good marrow aspirate you have much more than enough. So you can recommend your clinicians to be careful to give you the first 500ul aspirate which is definitely undiluted with the periphery. God bless On Thu, 8 May 2008 10:52:23 -0230, Ernest Stapleton wrote > We spoke to our clinicians about this problem and asked them how to minimize diluted samples. > The result was an awareness of the problems associated with blood dilution and left it up to them to decide whether to aspirate at 2 sites. The clinicians wants to minimize the discomfort to the patient balanced with the expectations of getting results for cytogenetics, molecular, flow cytometry and morphology on 1 draw. When made aware that their results might be compromised, they were very understanding. > > Best regards, > > Ernest Stapleton > Division Manager > Immunology and Genetics Laboratories > Room 1524 Health Sciences Center > St. John's, Newfoundland > A1B 3V6 Canada > 709-777-8654 > > -----Original Message----- > From: Rudensky, Bernard [mailto:rudensky@szmc.org.il] > Sent: Wednesday, May 07, 2008 9:06 AM > To: Cytometry Mailing List > Subject: Dilution of bone marrow > > > > Dilution of bone marrow with peripheral blood can greatly compromise the quality of the bone marrow sample and the subsequent FACS analysis. > I would greatly appreciate hearing how you manage to avoid or at least diminish the possibility of receiving these inadequate, potentially misleading specimens. > > Professor Bernard Rudensky > Clinical Laboratories > Shaare Zedek Medical Center > Jerusalem, Israel 91031 > > ----------------------------------------------------------------------- IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. > If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. > *** eSafe scanned this email for viruses, vandals, and malicious content. *** Shaare Zedek Medical Center http://www.szmc.org.il ----------------------------------------------------------------------- Looking forward to hearing from you, I remain Sincerely yours, Alireza Ardjmand, M.Sc Hematology, Oncology & stem cell Transplantation research Cent. Shariati Hospital Kargar Ave. 14114 Tehran-Iran http://www.horc-bmt.org Tel: +982184902626 Fax: +982188004140 Mobile: +989352335919 alternative email: ardjmar72@yahoo.comReceived on Mon May 12 14:38:00 2008
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