Dear colleagues, Can we count absolute cell number/concentration by simply dividing total cell count (or number of events collected) by the volume passed through the flow cytometer? The volume here is: (volume of counted cell suspension) = (volume in the tube before flow) - (volume in the tube after flow) - (volume of the dead space the flow Cytometer lines between the intake and the measuring chamber) If yes, how can we find/measure that dead space volume? Best regards, SergeiReceived on Fri May 9 13:38:00 2008
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