We spoke to our clinicians about this problem and asked them how to minimize diluted samples. The result was an awareness of the problems associated with blood dilution and left it up to them to decide whether to aspirate at 2 sites. The clinicians wants to minimize the discomfort to the patient balanced with the expectations of getting results for cytogenetics, molecular, flow cytometry and morphology on 1 draw. When made aware that their results might be compromised, they were very understanding. Best regards, Ernest Stapleton Division Manager Immunology and Genetics Laboratories Room 1524 Health Sciences Center St. John's, Newfoundland A1B 3V6 Canada 709-777-8654 -----Original Message----- From: Rudensky, Bernard [mailto:rudensky@szmc.org.il] Sent: Wednesday, May 07, 2008 9:06 AM To: cyto-inbox Subject: Dilution of bone marrow Dilution of bone marrow with peripheral blood can greatly compromise the quality of the bone marrow sample and the subsequent FACS analysis. I would greatly appreciate hearing how you manage to avoid or at least diminish the possibility of receiving these inadequate, potentially misleading specimens. Professor Bernard Rudensky Clinical Laboratories Shaare Zedek Medical Center Jerusalem, Israel 91031 _____ IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. *** eSafe scanned this email for viruses, vandals, and malicious content. *** Shaare Zedek Medical Center http://www.szmc.org.il _____Received on Thu May 8 15:18:00 2008
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