Thanks to all!

Dear Flow Experts:

     It is so good to know we are not alone! Thanks for all replies to my
e-mail. As my last sorting was so long ago (more that 6 month I believe) I was
so stressed! I run cell lines that were bigger than lymphocytes before using
the 70um nozzle at medium pressure, so I have the same template with voltages
for those cells, I change them a little but I didn't think it was that much
change and that was my mistake!!! After I started sorting I realized that cells
were going to the bottom so fast and shaking at 300 was not enough to keep them
in suspension, plus all the "rainy side stream" and I didn't have too many
cells to sort so to start again (changing nozzle, set the drop delay again and
do the sorting with the new settings). I didn't know these cells were so big!!!
I am sure we will not have any problem when I run them next time.
I needed your support! This mailing list really works! Thanks a lot Dr Robinson
for keeping it going. Thank you all: Charles, Steve, Pamela, Barbara, Dan,
Jerry, Charles, Debora, Katherina, Joseph for your concern and prompt reply!!!

All were coincident in their suggestions about the 100um nozzle and accudrop and
also about running accudrop after preparation for asceptic sort and then run
ethanol or bleach + sterile PBS to ensure sterility.

I appreciate your help very much!

Regards to all,

Vida