Hi all, I have an investigator wanting to do some calcium measurements on macrophages. I have scanned the literature and there seems to be several additives to help load the dyes or prevent them from being pumped out. I have found Probenicid, Pluronic F-127, PowerLoad and Sulfinpyrazone alone or in combination mentioned. Does anyone have any experience which works best, so we could maybe save a little time and money trying to figure it out? Also, does it matter what form you buy - water soluble or in DMSO? We will be trying the ratio metric measurement of fluo-3 and Fura Red AM on a FACSAria. Any other suggestions are welcome. Thanks in advance for your help! Pam Shaw Fluorescence Cytometry Core Facilitator Center for Environmental Health Sciences The University of Montana 32 Campus Dr, Skaggs Building 052 Missoula, MT 59812 Ph (406) 243-4974 email: <mailto:pamela.shaw@umontana.edu> pamela.shaw@umontana.edu www.umt.edu/cehs/fluor_main.html <http://www.umt.edu/cehs/fluor_main.html> <http://www.umt.edu/cehs/FluorCytomCore.htm>Received on Wed May 7 19:18:00 2008
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