Stimulating Human Bcells with CpG

 Dear all,

 I'm trying to stimulate human B cells with CpG ODN2006-G5.
 I stimulate PBMCs with 12uM CpG, during 48 hours. I'm analysing them at
 FACSAria
 and I'm using the following staining to differentiate Memory B cells and
 Plasma
 cells:

 CD19 - Pacific blue
 CD20 - APC Alexa Fluor 750
 CD38 - PE-Cy7
 CD138 - PE
 CD27 - APC
 IgD - Fitc

 Memory B cells phenotype: CD19+/CD20+/CD38+/CD138-/CD27+/IgD+
 Plasma cells phenotype:   CD19low/CD20-/CD38+++/CD138+/CD27hi/IgD-

 We didn't find any difference between stimulated and unstimulated cells.

 Do you know if CpG is adequate for Human B cell stimulation? Should I be
 using higher concentrations? Or am I looking at the wrong populations?


 Thank you,
 ines