Hi all, We are interested in doing some intracellular pH measurements and SNARF-1 AM seems to be the preferred dye. According to the literature, the ideal wavelengths to collect the two signals are at 580 and 640. On our Aria we have available a 575/26 or a 585/42 for the 580 signal. We have a choice of 695/40 or 675/20 for the 640 signal. Will we miss too much of this signal? We could do it on the Calibur which has a long-pass 650 filter, but the Calibur cannot do ratios of signals. Any suggestions? Has anyone else done this on an Aria? If you do this on the Calibur, how do you interpret the data? I looked on the archives, but was unable to find anything specific to the Aria. Thanks so much for your advice. Pam Shaw Fluorescence Cytometry Core Facilitator Center for Environmental Health Sciences The University of Montana 32 Campus Dr, Skaggs Building 052 Missoula, MT 59812 Ph (406) 243-4974 email: <mailto:pamela.shaw@umontana.edu> pamela.shaw@umontana.edu www.umt.edu/cehs/fluor_main.html <http://www.umt.edu/cehs/fluor_main.html> <http://www.umt.edu/cehs/FluorCytomCore.htm>Received on Mon May 5 15:58:00 2008
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