Hi all! I´m currently working in a model of acute pneumonia in mouse. I need to improve the FACS analysis of the BAL and single cell suspension we prepare from lungs. I´m having some trouble identifying alveolar macrophages because we´re using F480 but this marker is poorly expressed in lungs. I found a reference at the Journal of Immunology in wich they demonstrate that DECTIN 1 (also knokw as beta glucan receptor) is highly expressed by different types of macrophages including alveolar macs, but also in other cell types. (See reference at: *J of Immunol*, Taylor RP *et al*, 2002, 169: 3876-3882 or http://www.jimmunol.org/cgi/content/full/169/7/3876). FITC and PE conjugated anti-Dectin 1 markers are commercially available for flow cytometry. I wonder if I should use it because this marker is also expressed by neutrophils, some subsets of DCs and T cells. Have you ever tryed it? Can I use the FITC conjugated marker besides the high autofluorescence of the macrophages? Thank you in advance! Natalia -- Natalia Muñoz Wolf Laboratory for Vaccine Research, Department of Biotechnology, Instituto de Higiene, Facultad de Medicina Avda. A. Navarro 3051, Montevideo, CP 11600 Uruguay Tel.: + 5982 487 12 88 (ext. 1121) Fax: + 5982 487 30 73 email: nataliam@higiene.edu.uyReceived on Mon May 5 15:18:00 2008
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST
