Ficoll and tissue samples

I've a group who is layering minced, mesh-strained (no DNAse digestion)
human decidual tissue on Ficoll.  They are also using an adherence step
before Ficoll to deplete fibroblasts.

Could someone please tell me in which layer of the Ficoll separation I can
expect to find the human tissue macrophages and dendritic cells?  Will they
be in the buffy interface layer?

Thanks again,
Annie Bang.

		MOUNT SINAI SERVICES
		Annie Bang
		Flow Cytometry Centre
		Pathology and Laboratory Medicine
		Mount Sinai Hospital
		Room 6-209
		600 University Avenue
		Toronto, Canada  M5G 1X5
		Phone (1) (416) 586 4800 ext 6530
		Fax	(1) (416) 586 8628
 E-mail  abang@mtsinai.on.ca <mailto:kpritzker@mountsinaiservices.com> 

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