isolating monocytes - summary

From: Ruth Bates <ruth_louise-bates@hotmail.co.uk>
Date: Tue Apr 29 2008 - 11:30:13 EDT
Philip Barren (barvp@sbcglobal.net) wrote:
	    
	    
	
Check this out
 
www.stemcell.com/product_catalog/rosettesep.aspx
 
their products or tech support should be able to help
you


	
	
	     Nigel Miller (ngam2@mole.bio.cam.ac.uk) wrote:




    I frequently sort mono's and gate simply on FSC vs SSC. Works 
well,cheap and no messy colours.


Carolina Garcia de Alba Rivas (carogar7@gmail.com) wrote:
I've been working with a negative selection kit for CD14+ CD16-
monocytes, this kit it's from Stem Cell laboratory, easy sep technique,
I obtain a 90-95% purity and I really haven't observed activation
although I haven't properly analyze the cells for activation. They also
have kits for isolation of other populations and you might find
something useful.

 

					       
Ruth L Bates BSc(Hons) MSc PhD

"I'd rather die on my feet than live down on my knees"
Wayne Hussey 2007


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Received on Tue Apr 29 17:18:00 2008

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