Methods for fixing cells

Hi, everyone:
 
I'd like to fix my cells to run the flow later. The cells are human PBMC cells and are stained for surface markers only. PI will not be added and I will use FCS/SSC to gate out the living cells later. What kind of method I'd use? Can I use formalin instead of Para formaldehyde?  I tried 5% formalin but there lots dead cells after fixing. Thank you very much!
 
Qingyong Xu
Department of Pathology and Laboratory Medicine
Saskatoon Health Region and University of Saskatchewan
Canada.
_________________________________________________________________
Express yourself wherever you are. Mobilize!
http://www.gowindowslive.com/Mobile/Landing/Messenger/Default.aspx?Locale=en-US?ocid=TAG_APRIL