50 mW violet laser

Flowmeisters,

	In an effort to create redundancy across a couple of our LSR
II's we are looking at the addition of a 405 violet laser to one that
lacks such.	One of the machines that does have a violet laser has a
Coherent 25 mW.  In a quote the we received from BD they specified a 50
mW laser.  When asked about the fact that we wanted the 25 mW version
the response we received was that they weren't sure if they still
offered a 25 mW version.	That aside, this situation has raised a couple
of questions that I am hoping someone on the listserve could address.

	Our rationale for wanting the 25 mW rather than the 50 is that
higher power of the 50 will create a greater difference in signals
generated, thereby moving away from our goal of redundancy across
machines.  While no two machines will ever be exactly the same, and
proper controls and data analysis would be paramount we want to be
prepared to allow our users to switch between machines in the hopefully
rare case of there being no other choice.	In your opinion does the use
of a 50 vs a 25 create more potential disparity than both machines
having 25's?

	Secondly, does anyone out there have experience with the 50 mW
laser?  Is it more problem prone?  I guess the general question would be
what are the pluses and minuses both in terms of performance and
reliability with regard to the stronger laser?  Thanks in advance for
your input.

Dave






David Dunaway
Flow Cytometry Core Laboratory
The Research Institute at Nationwide Children's Hospital
700 Children's Drive
Columbus, Ohio 43205
(phone) 614-355-2820
Dave.Dunaway@nationwidechildrens.org



----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.