Re: CD25+ cells in unstimulated PBMCs

In normal PBMC (without apparent pathological conditions), the frequency
of CD25+ cells among CD4+ cells is less than 10%. This is considered as
the naturally occurring Treg population. After 3 days of stimulation with
a T cell mitogen like PHA, 65 to 80% of CD4+ cells will express CD25,
indicating T cell activation.

-- 
S. Jayaraman, Ph.D.
Associate Professor of Surgery
University of Illinois at Chicago
909 South Wolcott Avenue, COMRB 8113
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On Mon, April 14, 2008 5:41 pm, Howard Shapiro wrote:
>
>
> Olga Nikolaitchik wrote:
>> We see a large number of CD25+ cells in unstimulated PBMCs from a
>> healthy control donor.
>>
>> I wonder if something wrong with our donor, or is this a common
>> situation seen by other people, too. What is the "normal" percent of
>> CD25+ cells in resting PBMCs?
>>
> How large is "a large number"? When we were looking at lymphocyte
> activation in the mid-1980s, with bulk separated T cells, we would
> typically see no more than about 1.5% bearing what we now call CD25 or
> other activation antigens in unstimulated samples.
>
> -Howard
>
>