Hello all, an now for a science question..... A colleague of mine wants to look at CD117 expression on an adherent human cell line, but this antigen is sensitive to trypsin. Unfortunately, EDTA alone isn't enough to get these cells off the plastic and physically scraping them off would be our last resort. Has anyone successfully used an alternative cell-detaching agent (eg. accutase, liberase etc.) while retaining expression of this antigen? many thanks in advance, Sian. Dr.Sian Rizzo *NOTE RELOCATION on 1st APRIL 2008* to Office 113, McElwain building Prostate Stem Cell Laboratory Male Urological Cancer Research Centre Institute of Cancer Research 15 Cotswold Road Sutton Surrey SM2 5NG TEL: +44(0)20 8722 4004 email: Sian.Rizzo@icr.ac.uk The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addressee only. If the message is received by anyone other than the addressee, please return the message to the sender by replying to it and then delete the message from your computer and network.Received on Tue Apr 1 12:18:00 2008
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