Hello, I have troubles with the LoVo cell line : I can't avoid them clumping. I detached them using trypsin or EDTA. EDTA seems to work better but still, I have clumps. I tried to resuspend them several times, filter them, .... I can't find a way to avoid detach the cells from each other. This phenomenon brings 2 problems : 1) for the cell culture : they grow like "wine grapes", with small round cells in the middle and well-spread cells on the edge 2) for the cell staining and flow cyto analysis : I want to get rid of these clumps. Does anybody have clues or hints to get rid of clumps? Thanks a lot. JulieReceived on Tue Apr 1 11:38:00 2008
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