Dear all, I have consistently seen a background shift when using Annexin as a marker for Apoptosis. Some of the reasons we've come up with are 1) too high titre of antibody 2) rough handling when lifting adherent cell types 3) rough handling during staining /spinning Does the list have any thoughts on this ? thanks ralph Regards Ralph Rossi Flow Facility Manager Peter MacCallum Cancer Centre Melbourne , Australia Email: ralph.rossi@petermac.org Ph 96563747 , 96561955 This email (including any attachments) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt.Received on Wed Mar 26 14:38:00 2008
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