Hi Ahmed, are you primarily interested in a Standard Operating Protocol for deployment in a Clinical Flow facility, or are you more interested in the 'Research' aspects of CD34+ cell analysis? Once you have indicated what you need to do, we can help you a lot more efficiently. best, rob sutherland UHN Toronto On 18-Mar-08, at 8:26 AM, ahmed el wakeel wrote: > > hi all > i have FACS CALIBURE FLOWcutometer without sorter, > i need to use this devise in stem cell quantitative to identifing > the percentage of stem cell in my samples( whool blood and bon > marrow ) > pls i need acomplete prtocool for this application starting from > separation of mononuclear cells to preparing to intoducing to flow? > I have CD 34+ PE labelled > AC 133 pure CD133 CALIBRITE BEADS 2 COLORS > FACS LYSING SOLUTION > ISOTYPE CONTROOL ANTI MOUSE IgG > Isotype control Mouse Ig G1 PE > > hich can i use for this applcation: send aprotocol( complete) > thanks > > ahmed > > > لاإله إلا الله محمد رسول الله > Ahmed EL-wakeel > Flowcytometry Applications Specialist > Stem cell Research Center > Mobil: 0101046053 > > Connect to the next generation of MSN Messenger Get it now!Received on Thu Mar 20 14:38:00 2008
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