Hello Timothy... The Spectra-Physics, Cobolt and JDSU 488 nm modules are all excellent lasers with good noise, stability and beam shape characteristics. The current JDSU units are not quite as powerful, maximum 20 mW - fine for a cuvette instrument, but less than ideal for jet-in-air. But with the Elite flow cell design, maybe this is enough (?) - not sure. The Spectra-Physics Newport Cyan, formerly Picarro, is 50 mW - we have an early version of this module on our LSR II, it works well. Their website also lists their new Excelsior line, which claims to peak at 100 mW for 488 nm. The Cobolt Calypso is also more powerful, 100 mW I think. All of these lasers will require some laser mount design on your part to adapt them to your instrument - they are OEM modules that are not specifically set up for flow. ICyt Visionary Bioscience sells a Coherent Sapphire 200 mW module packaged in a mounting assembly, with fan, power supply and control software that will adapt to most cell sorters. Ours has worked well on our FACSVantage for the last four years. It's more expensive, but is a good drop-in solution for replacing an argon. Enjoy, Bill Usual disclaimer: I have no financial relationship with any of the above firms. -----Original Message----- From: Timothy Overton [mailto:two812@bham.ac.uk] Sent: Fri 2/29/2008 11:03 AM To: cyto-inbox Subject: Solid state replacements for an Epics Elite Hello all, I'm looking to replace the (failed) 488nm Argon ion laser in my Epics Elite with a solid state laser. Does anyone have any experience of the Spectra-Physics Cyan, Cobolt Calypso 491 nm or JDSU CW 488nm solid state units? Thanks, Tim Overton ***************************************** Dr Tim Overton Lecturer in Biochemical Engineering Chemical Engineering School of Engineering The University of Birmingham Birmingham B15 2TT t: +44 (0) 121 414 5306 e: t.w.overton@bham.ac.ukReceived on Mon Mar 3 11:58:00 2008
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