markers for granulocytes and monocytes in human PBMCs

Hi all,

I need to check the percentange of CD14-monocytes and
granulocytes after Ficoll of human whole blood, which
always consists of a small percentage of granulocytes
in my samples. I search the forum and see a post
talking about using CD15,CD16 for staining, together
with CD14. Since I use 2.4G supernatant to block the
non-specific binding of mouse IgG to the human cells 
CD16 might not be a good choice for me. I wonder if
any one has more comments about CD15/CD14, CD16/CD14
staining or further information about this topic.

Thank you very much in advance,

Li


     
____________________________________________________________________________________
Be a better friend, newshound, and 
know-it-all with Yahoo! Mobile.  Try it now. 
http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ