Dear Michelle, It would be helpful if you could attach a plot of your cells. A good positive control for apoptosis is serum starved or camptothecin treated Jurkat cells (http://www.nature.com/cdd/journal/v9/n5/full/4400996a.html). Serum starvation for 36 hours or camptothecin treatment (4microM for 3-4 hours) are good inducers of apoptosis in Jurkat cells. These treated Jurkat cells should show a good sub G1 peak. Vivek Tanavde Bioinformatics Institute Singapore Subject: Looking for subG1Date: Tue, 19 Feb 2008 10:24:02 +0800From: michellemok@bsf.a-star.edu.sgTo: cytometry@flowcyt.cyto.purdue.edu Dear flowers, I have a customer here performing analysis on my LSR II looking for a sub-G1 population. Trouble is, we haven’t seen any. So we’re not sure if his treatment protocol has an issue (still in optimization) or that the cells really do not have a sub-G1 population. So is there is a “gold-standard” cell line know for exhibiting a sub-G1 population under a certain treatment protocol? Any help would be most appreciated. Thank you, Michelle Mok Research Officer Flow CytometryReceived on Wed Feb 20 23:58:00 2008
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