Re: Facs Aria Threshold rate problem

Hi

I am sorting mouse spleen cells using FACS Aria and have never had  
any problems regarding unstable threshold. I have my cells in PBS, 10  
million cells/ml and have always mixing and cooling on when I start.  
If you have more cells than 10 million/ ml it may happen that they  
will fall down. Hope this helps and I will be happy to help more if  
this dose not help.

Regards

Sylvie Amu, PhD student
Dept of Rheumatology and Inflammation Research
Gothenburg University
Sweden

On Feb 12, 2008, at 3:27 PM, Joey Ilas wrote:

>
> We are sorting spleen cells from mouse and  we have problem with  
> the unstable threshold
> rate during sorting ( events rate goes down after few minutes of  
> sort ).
>
> We replaced the sample tubing and  tried to add EDTA or replace the  
> buffer with PBS  but
> no success.
>
> What could be the problem .
>
> regards ,
>
> Mr. J. Ilas
> -- 
> Psssst! Schon vom neuen GMX MultiMessenger gehört?
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