Hi Andrea: Try Accumax, works great in our hands for a variety of clumpy cell lines. http://www.innovativecelltech.com/accumax.html I believe you can get a free sample to try for the price of shipping. Joanne Lannigan, M.S. Director, Flow Cytometry Core University of Virginia Jordan Hall Room 7065 1300 Jefferson Park Avenue Charlottesville, VA 22908-0734 flowcytometry@virginia.edu (434) 924-0274 Office (434) 982-1071 Fax On Feb 8, 2008, at 2:41 PM, Andrea Bree wrote: > We are trying to FACS analyse LMH cells (a chicken hepatoma, > epithelial cell line). They are extremely clumpy. We have tried > trypsin, EDTA, EGTA, trypsin-EDTA ,Ca/Mg free buffer, passing the > cells through a prefilter, but the cells still clump terribly. Any > suggestions? > Andrea Bree > Wyeth Research
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