RE: Proving clonality on B cells

Hi Brian

 

It is not at all unusual to develop clear clonality by permeabilizing sIg- CLLs,
as well as other putative sIg- tumors.

It is neither counter nor non-productive!  Do it.

 

Fred

 

 

 

Frederic Preffer Ph.D.

Massachusetts General Hospital

Associate Professor of Pathology

185 Cambridge Street -	CPZN-4226

Boston MA 02114

________________________________

From: Marsiglia, Brian [mailto:Brian.Marsiglia@inova.org] 
Sent: Wednesday, February 06, 2008 12:28 PM
To: cyto-inbox
Subject: Proving clonality on B cells

 

I currently have three sets of K/L reagents.  Our protocol asks us to set up a
different vendors K/L if the B cells are surface negative.  If this second set
is also surface negative, then we call the sample surface Ig negative.	It has
been suggested that I set up cytoplasmic K/L on all negative cases.  This also
goes for CD19+/CD5 positive cases, as well as CD19+/CD10+.

 

I have always been under the impression that this is (a) non-productive, and
(b)counter-productive.	That there is no good information obtained by
cytoplasmics on CLL samples etc..

 

I would love some feedback on this issue.

 

Brian Marsiglia MT (ASCP)


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