We have our Aria now for 6 month running. So far we observed no contamination (or we are not told by our users / independent steril controls are not established yet). We run only steril PBS through our sheath lines and do a Long Clean with EtOH (70%) every morning followed by the normal Fluidic Start up. During long clean, we do not bypass the saline filter of the system. At the beginning, we did the long clean at the end of the day, but we got better stability and performance doing it every morning. So we get also rid of air bubbles, which might have formed over night. Our experience with the FACSVantage and DIVA is, that it also helps to run the machines daily or prevent longer times without usage. So even if we have no sorts (which happens not so often), we perform just a startup. So the longest time, the machine stands still is over weekend. As a side effect, we have to change 2-3 times per week the sheath tank. Not much time for a contamination to establish in a previously steril PBS tank (At least I hope this last very long). Good luck Steffen -- Dr. Steffen Schmitt Head of Flow Cytometry Core Facility DKFZ German Cancer Research Center (Deutsches Krebsforschungszentrum) Member of the Helmholtz Association Core Facility Flow Cytometry W220 Im Neuenheimer Feld 280 D-69120 Heidelberg Phone: 49-(0)6221-42-1261, -1262, -3740 E-Mail: steffen.schmitt@dkfz-heidelberg.de Web: www.dkfz.de Confidentiality Note: This message is intended only for the use of the named recipient(s) and may obtain confidential and/or privileged information. If you are not the intended recipient, please contact the sender and delete the message. Any unauthorized use of the information contained in this message is prohibited.Received on Fri Feb 1 11:38:00 2008
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