Hi Phil- I use dH2O and I've seen no ill effects compared to standard sheath fluid, or any particular contamination issues etc- we look at standard human and murine cells- maybe it may become an issue with other cell types or bacteria etc. Also, I fill the 20L Sheath Container provided by Dako so there are obviously no sensor or connections issues. All the best Barry. Barry Moran Research Officer and Manager Flow Cytometry Facility School of Biochemistry and Immunology Lab 0.19, Biotechnology Building Trinity College Dublin Ireland +353 1 896 3575 barry.moran@tcd.ie http://www.tcd.ie/biochemistry/flow _____ From: McCoy, J. Philip (NIH/NHLBI) [E] [mailto:mccoyj@nhlbi.nih.gov] Sent: 28 January 2008 17:50 To: cyto-inbox Subject: Sheath fluid for CYAN ADP What sources of sheath fluid are most people using for the CYAN ADP? Dako is having problems with their supplier and we are looking for alternative sources. If another vendor is used, how do you handle the sheath sensor (scale) and connections? thanks Phil J Philip McCoy, Jr., PhD Head, Flow Cytometry Core NHLBI - NIH 10 Center Dr, MSC 1357 Bldg 10, Rm 8C103D Bethesda, MD 20892 tel 301-451-8824Received on Wed Jan 30 13:38:00 2008
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