Re: mAbs to distinguish mouse macrophages and monocytes??

hello,

if you want to specifically target alveolar mature macrophages, a good 
parameter is - in my opinion - CD11c(high) / autofluorescence(high) / 
CD11b(med)
immature macrophages/monocytes will then be CD11c(+/med) / 
autofluorescence(low) / CD11b(+/med)
Of course, depending on the type of inflammatory stimulus, some changes 
can occur in the expression pattern of surface markers, but I think that 
this panel covers most of the conditions.

see also:
Accurate and simple discrimination of mouse pulmonary dendritic cell and 
macrophage populations by flow cytometry: Methodology and new insights
Vermaelen, K & Pauwels, R
Cytometry 2004

and

Dynamics of macrophage cell populations during murine pulmonary tuberculosis
Gonzalez-Juarrero M, Shim TS, Kipnis A, Junqueira-Kipnis AP, Orme IM
J Immunology 2003

** 
<http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Gonzalez-Juarrero%
20M%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVCitati
on>Hope 
this helps you somewhat further...

Pieter




Gwinn, William (NIH/NIEHS) [F] wrote:
>
> What is the ‘current’ recommendation regarding the best mAb panel to 
> distinguish _mouse_ tissue macrophages (specifically, alveolar) from 
> immature macrophages/monocytes by flow?
>
> Thank you.
>
> William M. Gwinn, Ph.D.
>
> IRTA Postdoctoral Fellow
>
> National Institute for Environmental Health Sciences
>
> gwinnwm@niehs.nih.gov
>
> Phone: 919-541-5564
>


-- 
Pieter Bogaert
Molecular Immunology Lab
Ghent University (UGent)
Fiers-Schell-Van Montagu' building
Technologiepark 927
B-9052 Ghent (Zwijnaarde), Belgium

Tel :  +32-9-33 13 655
e-mail : pieter.bogaert@dmbr.UGent.be