hi do you mean 1N vs 2N genomes per cell (and <1 and >2) ? If so, this is a standard cell cycle analysis and cells fixed, permeablized and then treated with PI is the "gold standard" and I am sure you can easily find protocols. There are a number of alternative fluors as well, including for live cell analysis. ======================================================= Rachel M. Gerstein, Ph.D. Associate Professor Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) -----Original Message----- From: Sripriya Subramaniam [mailto:sripriya@vasci.umass.edu] Sent: Thu 1/17/2008 4:42 PM To: cyto-inbox Subject: Quantifying genome DNA Hello everybody, I have a student who is looking to quantify entire genomic DNA by flow cytometry. Has anyone worked on this in the past ? Any suggestions will be greatly appreciated. - Sripriya Subramaniam Flow cytometry facility University of MassachusettsReceived on Tue Jan 22 11:58:00 2008
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