Date: Fri Jan 18 2008 - 03:17:49 EST
There is a tutorial (and sample files) by FlowJo available at http://www.flowjo.com/home/tutorial.html It takes you through the steps of titrating a FITC labelled anti-CD8 reagent to determine the most economical concentration for staining. Kind regards John Michie +27 21 9389 539 +27 21 933 8886 083 449 2225 -----Original Message----- From: Abdul Khan [mailto:khan.abdulh@googlemail.com] Sent: 17 January 2008 12:10 AM To: cyto-inbox Subject: Titrating the antibodies Dear All, Is it necessary to titrate all the antibodies before doing the FACS analysis, eventhough most of my antibodies are pre-titrated/pre-diluted (as per datasheet) but some of them say only concentration like 0.2 mg. I have got 15 antibodies and would appreciate any help/advice in this regard. Can anyone please suggest the best way to titer the antibodies, I will be doing 3 color flow cytometry. I would also like to know if it is best to buy the antibody and isotype control from the same company ? I bought one antibody from a different company but I already have the isotype control from another company, now is it recommended and worth buying the isotype control from the same company from where I bought the antibody or shall I buy one ? I shall look forward to your responses. Thanks a lot. Best wishes, Abdul
