You may try CD34 APC in BD-Biosciences http://biocompare.net/itemdetails.asp?itemid=400514 Quoting Jos Bosch <j.a.bosch@bham.ac.uk>: > Dear Flowers, > > > > Am setting up a panel on a 6-color FACScanto to characterize stem cells > in peripheral blood, and running into the usual planning problem on what > fluorochromes to assign to particular antibodies. The main hassle is > caused by the fact that CD133 (miltenyi) and KDR (R&D) are only > available in PE and APC, so have to work around these. > > > > To make a long story short, there are two options I seem to be left > with: > > 1) Substitute CD34 PE (clone 8G12, BD) for PerCP. > > Arseniev (1999) concluded PerCP was not really bad, but not really great > either. What is your experience?? Was unable find any systematic > comparison in the literature that looked at PE-Cy7 or APC-Cy7. Does > anyone have good validation data? > > 2) Alternatively, I can buy CD133 biotin and label it with FITC. > > Does anyone have good experience with that approach? What can I expect > with regard to signal strength, background, and prep hassle? > > > > Your advise is very much appreciated! > > > > Kind wishes, > > > > Jos > > > > Jos A. Bosch, PhD > > Lecturer in Exercise and Behavioural Immunology > > School of Sport and Exercise Sciences > > University of Birmingham > > Birmingham B15 2TT > > United Kingdom > > > > T: (+44) 0121 4148105 > > F: (+44) 0121 4144121 > > http://www.sportex.bham.ac.uk/staff/boschja > > > >Received on Fri Dec 21 11:58:00 2007
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