Plating after annexin sorting

From: Moysés miranda <moyses_m@yahoo.com>
Date: Wed Dec 12 2007 - 07:34:21 EST
Hello everybody,
does anyone have experience with cell plating after
sorting for annexin positive and negative cells?
Can annexin positive sorted cells survive?
I have been sorting bovine fibroblasts induced with
staurosporine in FACSAria. Annexin negative cells are
living after plating and, surprising for us, positive
cells are surviving as well. Is it normal?
regards
Moyses Miranda
Developemental Molecular Laboratory 
Sao Paulo State University - Brazil



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Received on Wed Dec 12 11:38:00 2007

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