[ Live DNA dye with 633 excitation? ]

Dear Dr. Vámosi,

Have you heard of the Vybrant DyeCycle Dyes out of Molecular Probes / Invitrogen?  They
are really optimal for live cell cycle analysis.  You can use the dyes to analyze, sort
and re-grow cells.  Although we do not currently have a 633 excitable version we do have
a 532 excitable version, please see:

http://probes.invitrogen.com/lit/bioprobes49/bp49_01.pdf

http://probes.invitrogen.com/lit/bioprobes52/BP52_05.pdf

Regards,

Mike Olszowy 

-----Original Message-----
From: Vamosi Gyorgy [mailto:vamosig@dote.hu] 
Sent: Tuesday, July 24, 2007 6:44 AM
To: cyto-inbox
Subject: [ Live DNA dye with 633 excitation? ]

Dear All,

I would be interested in using a DNA binding dye 
with 633 nm excitation and live staining 
conditions. By now I have tested DRAQ5, but found 
some unspecific binding to proteins in addition 
to DNA binding. I would also be interested in 
other red dyes with 532 excitation that do not 
overlap at all with the FL1 channel on a flow 
cytometer. (PI and EB have relatively large 
overlaps with the green channel, so they are no good).

Many thanks,

György Vámosi, Ph. D.
Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences
Department of Biophysics and Cell Biology
Medical and Health Science Center
University of Debrecen

Nagyerdei krt. 98.
Debrecen
H-4012 Hungary

Tel./fax: +36-52-412-623
email: vamosig@jaguar.dote.hu