Hi Flowers, I am new to DNA analysis by flow so please bear with me. Can anyone tell me how I can tell the difference between 2N cells that are in G2/M phase and 4N cells in G0? On my gated histogram they fall on top of each other, correct? With my positive control, I can see a very small 8N peak that tells me that I have hyperploid cells. In my experimental condition, however, I am unable to say if the peak I see is only 2N cells undergoing regular cycling in G2/M, or if it is a G0/G1 peak of 4N cells. I'm guessing that the only way to distinguish this is to look for additional peaks representing 4N cells in S or G2/M (or 8N peaks, etc) but thought I would see if there's anything I'm missing. Thanks, Kerry Kerry.White@ipi.com This message and any attachments may contain confidential information and/or privileged material. If you received this message in error, please contact the sender and delete this message and any attachments immediately. Thank you.Received on Tue May 1 11:18:00 2007
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