Dan, I agree that this is likely a thermodynamic (temperature) phenomenon. I suspect that the elongation of the drop break-off is due to less efficient conduction of the drop drive pulse by the sheath fluid, which becomes less dense as it warms from the increasing ambient temp. When faced with this same problem on our MoFlo, I adjusted both the phase and increased the amplitude of the DD to maintain the original break-off distance - successfully finishing the sort and maintaining high purity of the sorted populations. I'm not sure if this strategy would work on your Aria though. Jim ------------------------------ James M. Simone Section Leader Flow Cytometry Section Office of Science and Technology NIH/NIAMS BLDG10 RM6D57 MSC1560 10 Center Drive Bethesda, MD 20892-1560Received on Wed Apr 25 12:38:00 2007
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