We always used only CD33-APC in 4-color panels, now after moving to 6-color panels we are keeping CD33-APC and adding CD33-PerCP-Cy5.5. We are talking all BD reagents. So far works fine and even weak CD33 staining is separated from negative populations. Irina Irina Grigorieva, PhD Director, Flow Cytometry Laboratory Northside Hospital, Atlanta, GA (404)- 851-6541 e-mail: irina.grigorieva@northside.com > -----Original Message----- > From: Dalal, Bakul [Dr.][VA] [SMTP:Bakul.Dalal@vch.ca] > Sent: Monday, April 02, 2007 3:37 PM > To: Cytometry Mailing List > Subject: Weak CD33 > > In your experience, which CD33 with which fluorochrome is brightest? > > We have a perpetual problem with weak CD33 (used with PE in past, now with PECy7). Especially evident when you have low CD33 AMLs. > > > Many thanks > > > Bakul > > > > Bakul I. Dalal MD FRCPC FCAP FASCP > Clin Prof, Faculty of Medicine > Dvn Hempath, Vancouver General Hospital > Vancouver BC, Canada > 604 875 4496(v)/4798(f)/8775949(p)/4846005(r) > bakul.dalal@vch.ca <mailto:bakul.dalal@vch.ca> > > Change is inevitable, except from a vending machine. > >Received on Wed Apr 4 10:58:00 2007
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